Anti-biofilm activity of LC-MS based Solanum nigrum essential oils against multi drug resistant biofilm forming P. mirabilis

Abstract Urinary tract infections are second most important diseases worldwide due to the increased amount of antibiotic resistant microbes. Among Gram negative bacteria, P. mirabilis is dominant biofilm producer in urinary next E. coli. Biofilm a process that produced self-matrix more virulence pathogens on colloidal surfaces. Based above fact, this study was concentrated inhibit formation by various in-vitro experiments. In current study, anti-biofilm effect essential oils recovered from medicinal plant Solanum nigrum, and confirmed available liquid chromatography-mass spectroscopy analysis. The excellent anti-microbial activity minimum inhibition concentration against indicated at 200 µg/mL. absence viability altered exopolysaccharide structure treated cells were showed metabolic assay phenol–sulphuric acid method. fluorescence differentiation with damages confocal laser scanning electron microscope. Further, morphological changes differentiated normal Altogether, results reported S. nigrum have ability.